Course Content
TRANSPORT IN PLANTS AND ANIMALS
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EXCRETION AND HOMEOSTASIS
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Biology Form 2
About Lesson
Practical Activity: To show that heat is produced during aerobic respiration

 

Requirements

  • Bean seeds
  • Disinfectant
  • Vacuum flask
  • Thermometer

 

Procedure
  1. Soak some bean seeds for a day and wash them in dilute disinfectant.
  2. Place the germinating seeds into a vacuum flask and set as shown in the diagram.
  3. Read off the initial temperature and record.
  4. Leave the vacuum flask in an inverted position for two days.
  5. Note the temperature change at the end of the two days.
Results and Explanation
  • The process that was being investigated was respiration.
  • The expected results is that there would be a rise in temperature because the germinating seeds were respiring releasing heat energy.
  • The vacuum flask was used to prevent any heat loss from the flask or heat gain from the surrounding.
  • The cotton wool was used instead of a rubber ring to allow diffusion of gases into and out of the flask.
  • The seeds were disinfected to kill any bacteria and fungi on the seeds thus preventing heat production from their respiration.
  • The vacuum flask was inverted to allow the heavier carbon (IV) oxide diffuse out of the flask and give room for lighter oxygen to diffuse in.
  • A suitable control experiment for this is, is having used boiled seeds instead of live ones in the vacuum flask.